表 2. The sequence of primers for for qRT-PCR.

定量引物序列

Gene	Forward primers (5′→3′)	Reverse primers (5′→3′)
HADHA: Hydroxyacyl-CoA dehydrogenase alpha subunit; ASCL2: Achaete-scute family BHLH transcription factor 2; MMP: Matrix metalloproteinase; HLA-G: Human leukocyte antigen-G; NCAD: N-cadherin; TNC: Tenascin C; RELN: Reelin; FGF1: Fibroblast growth factor 1; CCND1: Cyclin D1; LAMA2: Laminin subunit alpha 2; MEP1B: Meprinasubunit beta; IGFBP5: Insulin like growth factor binding protein 5; STK31: Serine/threonine kinase 31; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
HADHA	CTGCCCAAAATGGTGGGTGT	GGAGGTTTTAGTCCTGGTCCC
ASCL2	CTCGACCTATGAGCCTCAG	AATCTCCAAGTGTTGGTGC
MMP2	ATACCATCGAGACCATGCG	ATACCATCGAGACCATGCG
MMP9	TGCAACGTGAACATCTTCG	GAATCGCCAGTACTTCCCA
HLA-G	TGAGATGGAAGCAGTCTTCC	TGAGATGGAAGCAGTCTTCC
NCAD	CCTGCTTATCCTTGTGCTGA	CCTGGTCTTCTTCTCCTCCA
TNC	CATCGGAAAGATTTCTATAGACACC	AACTGAAGTGGCTTCAGCA
RELN	AAAGGAGTCTTACTGCGCT	TTTGTTTGCGAGTGAGGAC
FGF1	TATACGGCTCACAGACACC	TCTCTGCATGCTTCTTGGA
CCND1	CATTGAACACTTCCTCTCCA	AACTTCACATCTGTGGCAC
MMP1	ACAGTAAGCTAACCTTTGATGC	TTGTGCGCATGTAGAATCTG
LAMA2	AGTTCTGACACATGGTCCT	TTTCTTGAAAGCCCGAACTG
MEP1B	AAACTTTGATGTAGATGGCGG	TCAAGTCTGATGTCACCCTC
IGFBP5	GAGCAAGTCAAGATCGAGAG	CTTCTTCACTGCTTCAGCC
STK31	TTCAGTTTTGGGGAATCTTGACC	ACCAGACACTTTTCAACGCTG
GAPDH	AGATCATCAGCAATGCCTCCT	TGGTCATGAGTCCTTCCACG