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. 2022 Jan 20;53(1):127–132. [Article in Chinese] doi: 10.12182/20220160106

表 1. The 14-color panel for flow cytometry of leukocytic subsets in human peripheral blood.

14色流式检测人外周血白细胞亚群方案

Specificity Fluorochrome Clone Purpose
 NK: Natural killer; DC: Dendritic cell.
CD45 FITC HI30 Leukocytes
CD3 BV510 SK7 T cells
CD4 Qdot 655 S3.5 CD4 T cells
CD8 PE-Cy7 RPA-T8 CD8 T cells
CD19 BV421 HIB19 B cells
CD56 BV605 NCAM16.2 NK cells
CD16 BUV395 3G8 NK cells/neutrophil cells
CD14 Percp-Cy5.5 M5E2 Monocytes
CD25 APC M-A251 Treg
CD127 PE A019D5 Treg
HLA-DR APC-Cy7 L243 DC
CD123 BV711 9F5 Plasmacytoid DC/
basophil
CD11c APC-R700 3.9 Myeloid DC
Viability DAPI Dead cells