表 1. Primer sequence in PCR.

PCR引物序列

Gene	Sense	Anti-sense
AURKB: Aurora kinase B; BUB1: BUB1 mitotic checkpoint serine/threonine kinase; CDC20: Cell division cycle 20; CCNB2:Cyclin B2; CDCA5: Cell division cycle associated 5; CDK1: Cyclin dependent kinase 1; DLGAP5: DLG associated protein 5; EXO1: Exonuclease 1; KIF4A: Kinesin family member 4A; KIF2C: Kinesin family member 2C; NDC80: Nuclear division cycle 80; NEK2: NIMA related kinase 2; NCAPH: Non-SMC condensing Ⅰ complex subunit H; NUSAP1: Nucleolar and spindle associated protein 1; ORC1: Origin recognition complex subunit 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
AURKB	5′-CTGCACCATCCCAACATCCT-3′	5′-TGCCAACTCCTCCATGATCG-3′
BUB1	5′-TAAACCCACAGGAGCCAGGAC-3′	5′-CAAGCCTCAACGCCCAACT-3′
CDC20	5′-ATGCGCCAGAGGGTTATCAG-3′	5′-AGGATGTCACCAGAGCTTGC-3′
CCNB2	5′-CACAGGATACACAGAGAATG-3′	5′-CTTGATGGCGATGAATTTAG-3′
CDCA5	5′-GGCCAGAGACTTGGAAATGT-3′	5′-GGCCAGAGACTTGGAAATGT-3′
CDK1	5′-CCCTTTAGCGCGGATCTACC-3′	5′-CATGGCTACCACTTGACCTGT-3′
DLGAP5	5′-AAGTGGGTCGTTATAGACCTGA-3′	5′-TGCTCGAACATCACTCTCGTTAT-3′
EXO1	5′-TCTGAGAGGTAGTTAATTTGG-3′	5′-TACATCCATCAAATACGAGA-3′
KIF4A	5′-TCTGTTTCAGGCTGCTTTCA-3′	5′-GCCCTGAAATATTTGATTGGAG-3′
KIF2C	5′-GATGGAAGCCTGCTCTAACG-3′	5′-GAGCAGATTCCGCTTTGTTC-3′
NDC80	5′-CCTCTCCATGCAGGAGTTAAGA-3′	5′-GGTCTCGGGTCCTTGATTTTCT-3′
NEK2	5′-TGCTTCGTGAACTGAAACATCC-3′	5′-CCAGAGTCAACTGAGTCATCACT-3′
NCAPH	5′-GTCCTCGAAGACTTTCCTCAGA-3′	5′-TGAAATGTCAATACTCCTGCTGG-3′
NUSAP1	5′-CTGTGCTTGGGACACAAA-3′	5′-TTGTCAACTTGAATGGGGTAATAA-3′
ORC1	5′-ACGCAATGAAGTCTCTGGAATGTG-3′	5′-ACGGGCACGAAGAGAAGTATGGA-3′
GAPDH	5′-CATTCAAGACCGGACAGAGG-3'	5′-ACATACTCAGCACCAGCATCACC-3′