TABLE 3.
Postchallenge virological follow-up to determine whether sterilizing immunity was achieved
| Group | Animal | Results
obtained by PCR and QVI at wka:
|
Infection statusc postchallenge | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 2
|
4
|
6
|
12b | ||||||
| PCR | QVI | PCR | QVI | PCR | QVI | ||||
| gp120/DNA | I038 | + | 27 | + | 3 | + | 3 | + | Infected |
| Q062 | + | 0 | + | 81 | + | 9 | + | Infected | |
| I044 | + | 9 | + | 9 | + | 0 | + | Infected | |
| X007 | + | 0.5 | + | 27 | + | 0 | + | Infected | |
| rgp120/MF59 | I046 | + | 0 | − | 0 | − | 0 | − | Transient infection |
| T118 | + | 0 | − | 0 | − | 0 | − | Transient infection | |
| J041 | + | 0 | − | 0 | − | 0 | − | Transient infection | |
| Z64 | −d | 0 | − | 0 | − | 0 | − | Transient infection | |
| rgp120/ISCOM | T122 | − | 0 | − | 0 | − | 0 | − | Protected |
| L159 | + | 0 | − | 0 | − | 0 | − | Transient | |
| Q048 | + | 1 | − | 0 | − | 0 | − | Transient infection | |
| X009 | − | 0 | − | 0 | − | 0 | − | Protected | |
| DNA controls | Q045 | + | 9 | + | 0.5 | + | 1 | + | Infected |
| Q054 | + | 27 | + | 27 | + | 3 | + | Infected | |
| Subunit protein controls | EP4 | + | 3 | + | 9 | + | 27 | + | Infected |
| WK2 | + | 81 | + | 9 | + | 1 | + | Infected | |
Postchallenge virology was based on PCR of DNA from PBMC, with the results being scored as positive (+) or negative (−), and quantitative virus isolation (QVI), with values being the number of virus-producing cells per 106 PBMC. All PCR results were negative and all QVI values were 0 at week 0.
Shown are PCR results for antibodies other than gp120.
Protected, negative for virus by all parameters, including multiple nested PCR analysis of DNA from lymph node biopsy specimens; transient infection, positive at only one time point (week 2), by only one assay, and negative for virus by all parameters at all time points thereafter.
Positive plasma RNA signal detected only at week 2 (see Fig. 3).