KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-human CD24-APC, Clone 32D12 | Miltenyi | Cat#130–095-954 |
| Mouse anti-human CD90-FITC, Clone 5E10 | Biolegend | Cat#328108 |
| Mouse anti-human CXCR4-APC/Cy7, Clone 12G5 | Biolegend | Cat#306528 |
| Mouse anti-human EGFR-PE-Cy7, Clone AY13 | Biolegend | Cat#352910 |
| Mouse anti-human PDGFRA-biotin, Clone 16A1 | Biolegend | Cat#323504 |
| Mouse anti-human CD31-PE, Clone WM59 | BD Bioscienc es | Cat#563652 |
| Mouse anti-human CD34-PE, Clone 581 | BD Bioscienc es | Cat#562383 |
| Mouse anti-human CD45-PE, Clone HI30 | Biolegend | Cat#304010 |
| Mouse anti-human CD105-PE, Clone 266 | BD Bioscienc es | Cat#562380 |
| Mouse anti-human CD235a-PE, Clone HIR2 | Biolegend | Cat#306606 |
| Mouse anti-A2B5-APC, Clone 105HB29 | Miltenyi | Cat#130–098-039 |
| Mouse anti-human LIFR-PE, Clone 12D3 | BD Bioscienc es | Cat#566384 |
| Goat anti-human DCX, Clone C-18 | Santa Cruz Biotechnol ogy | Cat#sc-8066 |
| Mouse anti-human GFAP, Clone STEM123 | Takara | Cat#Y40420 |
| Chicken polyclonal anti-MAP2 | Abcam | Cat#ab5392 |
| Rabbit polyclonal anti-Synapsin 1 | ThermoFisher Scientific | Cat#A-6442 |
| Mouse anti-O4, Clone O4 | R&D Systems | Cat#MAB1326 |
| Mouse anti-human cytoplasmic protein, Clone STEM121 | Takara | Cat#Y40410 |
| Rabbit polyclonal anti-SOX2 | Abcam | Cat#ab97959 |
| Rabbit anti-OLIG2, Clone EPR2673 | Abcam | Cat#ab109186 |
| Rabbit polyclonal anti-NeuN | Abcam | Cat#ab104225 |
| Rabbit anti-EGFR, Clone EP38Y | Abcam | Cat#ab52894 |
| Goat polyclonal anti-human PDGFRA | R&D Systems | Cat#AF-307 |
| Mouse anti-Alpha B Crystallin antibody, Clone 1B6.1–3G4 | Abcam | Cat#ab13496 |
| Rabbit polyclonal anti-human HOPX | Sigma | Cat#HPA030180 |
| Rabbit polyclonal anti-ETV4 | ThermoFisher Scientific | Cat#PA5–76825 |
| BUV395 Streptavidin | BD Bioscienc es | Cat#564176 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Liberase | Roche | Cat#5401119001 |
| DNase I | Worthingt on | Cat#LS002007 |
| Accutase | Innovative Cell Technologies | Cat#AT104 |
| Polyvinyl alcohol | Sigma | Cat#P8136 |
| Poly-L-ornithine hydrobromide | ThermoFisher Scientific | Cat#P3655 |
| Laminin | ThermoFisher Scientific | Cat#23017015 |
| X-VIVO 15 media | Lonza | Cat#04–744Q |
| DMEM/F12 | ThermoFisher Scientific | Cat#11320082 |
| N-2 | ThermoFisher Scientific | Cat#17502048 |
| Heparin | STEMCELL Technologies | Cat#07980 |
| N-acetylcysteine | VWR | Cat#E-3710 |
| Fibroblast growth factor 2 | Shenando ah Biotechnology | Cat#100–146 |
| Epidermal growth factor | Shenando ah Biotechnology | Cat#100–26 |
| Leukemia inhibitory factor | Sigma | Cat#LIF1010 |
| Platelet-derived growth factor AA | Shenando ah | Cat#100–16-10ug |
| Biotechnol ogy | ||
| Insulin-like growth factor 1 | PeproTech | Cat#100–11 |
| Neurotrophin-3 | PeproTech | Cat#450–03 |
| B27 | ThermoFi sher Scientific | Cat#17504044 |
| Paraformaldehyde | Electron Microscopy Sciences | Cat#15710 |
| Normal goat serum | ThermoFi sher Scientific | Cat#50062Z |
| Triton-X | ThermoFi sher Scientific | Cat#85111 |
| RNase inhibitor | Clontech | Cat#2313B |
| UltraPure water | ThermoFi sher Scientific | Cat#10977015 |
| Tris-HCl pH 8.5 | Teknova | Cat#T5085 |
| Dithiothreitol | Promega | Cat#P1171 |
| GTP | ThermoFi sher Scientific | Cat#R0461 |
| Maxima H-minus reverse transcriptase | ThermoFi sher | Cat#EP0753 |
| KAPA HiFi HotStart ReadyMix | Kapa Biosystems | Cat#KK2602 |
| AMPure XP beads | Beckman Coulter | Cat#A63882 |
| Fast Green dye | Sigma | Cat#F7252 |
| Propidium iodide | Sigma | Cat#P4170 |
| ProLong Gold Antifade Mountant | ThermoFisher Scientific | Cat#P36934 |
| Chlorinated water | Innovive | Cat#M-WB-300C |
| Teklad global 18% protein rodent diet | Envigo | Cat#2918 |
| Teklad global 19% protein extruded rodent diet | Envigo | Cat#2919 |
| Critical Commercial Assays | ||
| LEGENDScreen™ Human PE Kit | Biolegend | Cat#700007 |
| Deposited Data | ||
| scRNA-seq data | This paper | PRJNA798712 |
| Original code | This paper | https://github.com/transcriptomics/NSPC |
| Experimental Models: Organisms/Strains | ||
| Mouse: NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) | The Jackson Laboratory | JAX: 005557 |
| Human: human fetal samples | Advanced Bioscience Resources | N/A |
| Oligonucleotides | ||
| Oligo-dT30VN (5’-AAGCAGTGGTATCAACGCAGAGTACT30VN-3’) | IDT | N/A |
| Template-switching oligonucleotide (TSO) (5’-AAGCAGTGGTATCAACGCAGAGTA CATrGrG+G-3’) |
IDT | N/A |
| ISPCR primers (5’-AAGCAGTGGTATCAACGCAGAGT-3’) | IDT | N/A |
| ERCC (External RNA Controls Consortium) ExFold RNA Spike-In Mixes | Invitrogen | Cat#4456739 |
| dNTP | Invitrogen | Cat#10297–018 |
| Software and Algorithms | ||
| ImageJ | NIH | http://wsr.imagej.net/distros/ |
| GraphPad Prism 9.0 | GraphPad Software | http://www.graphpad.com/scientific-software/prism |
| Fragment Analyzer Controller | Agilent | https://www.agilent.com/en/product/automatedelectrophoresis/fragment-analyzer-systems/fragmentanalyzer-systems-software/fragment-analyzersoftware-1149185 |
| Prosize | Agilent | https://www.agilent.com/en/product/automatedelectrophoresis/fragment-analyzer-systems/fragmentanalyzer-systems-software/fragment-analyzersoftware-1149185 |
| BD FACSDiva | BD Biosciences | https://www.bdbiosciences.com/en-us/products/software/instrument-software/bd-facsdivasoftware |
| FlowJo | FLOWJO LLC | https://www.flowjo.com/ |
| Mantis | Formulatrix | https://formulatrix.com/life-science-automationblog/liquid-handling-software-update-mantis-47/ |
| Mosquito | SPT Labtech | https://www.sptlabtech.com/products/mosquito |
| bcl2fastq2 2.19 | Illumina | https://support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software.html |
| Skewer 0.2.2 | Jiang et al., 201439 | https://sourceforge.net/projects/skewer |
| STAR 2.6 | Dobin et al., 201340 | https://github.com/alexdobin/STAR |
| RSEM 1.3.3 | Li and Dewey, 201141 | https://deweylab.github.io/RSEM/ |
| Scanpy 1.8.2 | Wolf et al., 201842 | https://scanpy.readthedocs.io/en/stable/ |
| Extreme Limiting Dilution Analysis | Hu & Smyth, 200938 | https://bioinf.wehi.edu.au/software/elda/ |