TABLE 1.

RT-PCR and sequencing primers

RNA amplified or sequenced	Primer sequence	Primer designation	Nucleotides
RT-PCR HuCV-229E N gene	5′-TCTGCCAAGAGTCTTGCTCG-3′	E7 sense	819–838a
	5′-AGCATAGCAGCTGTTGACGG-3′	E9 antisense	1035–1054a
RT-PCR HuCV-229E S1 gene portion	5′-GTTGCATATTGCTGGTTGTCAAAC-3′	229E-S1PH sense	68–91b
	5′-CCTGGCGCTATTTCTTAAGGC-3′	229E-S1PI antisense	1944–1964b
RT-PCR GAPDH gene	5′-GTGAAGGTCGGAGTCAACG-3′	GAPDH-H sense	10–68c
	5′-CACCTGGTGCTCAGTGTAGC-3′	GAPDH-I antisense	824–843c
Sequencing S1 gene portion	5′-GGAACCATTTTGTTTAAAACATC-3′	S-229E-A sense	426–448b
	5′-GTACGTTGACTTCAAACCTCAG-3′	S-229E-B sense	960–981b
	5′-CTCCTTGTAACCCACCAGATC-3′	S-229E-C sense	1522–1542b
	5′-GGTTACAAGGAGTGATAGAGTAG-3′	S-229E-D antisense	1511–1533b
	5′-CGTACAACACAATAAACGTATG-3′	S-229E-E antisense	933–944b
	5′-CAAAATGGTTCCACGTCTAAGG-3′	S-229E-F antisense	414–435b

^aReference 60. 

^bReference 55. 

^cReference 21.