Fig. 1.

Sam68 is highly expressed in calcified valve tissues. Representative image of Alizarin Red S A and immunohistochemical staining B of Sam68. C Quantification of immunohistochemistry results (n = 6). D and E Protein level of Sam68 in valve tissue measured by western blotting (n = 10). F Correlation analysis between AVC score and Sam68 protein expression (n = 10). G The gene expression of Sam68 and Runx2 were tested by qRT-PCR, the relevant level was normalized to GAPDH gene expression (n = 6). H Immunofluorescent staining of Sam68 (red) and Vimentin (green), DAPI (blue). magnification was 200 × and 400 × . *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001