Fig. 3.

Sam68 promoted osteogenic differentiation via STAT3 signaling pathway. A, B The p-p65/t-p65 and p-STAT3/t-STAT3 ratios and protein expression were detected as TNF-α treatment by time of 0, 2, 5, 10, 30, 60 min (n = 3). C Sam68 overexpression and STAT3 silencing efficiency. D, E and F ALP and Runx2 protein expression when silencing STAT3 with or without Sam68 overexpression, a negative vector (Vector) and siRNA (SiNC) were both used and quantification (n = 4). (G, H) Alizarin Red S staining and quantification (n = 3). ns. not significant difference, *p < 0.05, *p < 0.01, ***p < 0.001, ****p < 0.0001