Fig. 6.

Sam68 attenuated STAT3 phosphorylation by interacting with STAT3 SH2 domain. A Protein–protein interactions (PPIs) were predicted by using the online String database (www.string.com). B Predicted binding mode of Sam68 and STAT3 based on docking, the yellow dashed line represents the hydrogen bond interaction. stick indicates amino acids that form hydrogen bonds. C Immunoprecipitation was performed in hVICs. D Silver-stained gel of total protein from hVICs after immunoprecipitation with IgG or anti-Sam68 antibody. E STAT3 peptides were derived from the mass spectrometric analysis. F Immunoprecipitation analysis of hVICs with C188-9 and BP-1–102 treatment. G, H Binding mode of small molecules and proteins obtained by docking, an overall view and a partial view were both displayed, the blue stick in the picture is the small molecule, the light cyan Cartoon is the protein, the yellow dotted line indicates hydrogen bonding, and the gray dotted line indicates hydrophobicity effect. I The schematic diagram of competitive binding inhibitors