TABLE 3.

Characterization of sequence changes in S1 during persistent HCV-OC43 infection

Cell line(s)	Infection	Position of mutationa	Codon change	Amino acid change
HRT-18, H4, U-87 MG, U-373 MG, and MO3.13	Acute and persistent (all clones)	894–895b	GAA→GTT	E→V
		949b	GCG→GCA	No change
		2025b	ATA→ACA	I→T
HRT-18	Persistent (passage 42)	279c	AGT→ACT	S→T
		1496	AAT→CAT	N→H
		1740cf	CGA→CAA	R→Q
H4g	Persistent (passage 36)	102e	GAT→GCT	D→A
		134c	CCT→TCT	P→S
		579b	CAT→CGT	H→R
		752	TAT→CAT	Y→H
		1445cf	CTT→TTT	L→F
U-87 MG	Persistent (passage 28)	102c	GAT→GTT	D→V
		110f	AAT→TAT	N→Y
		279c	AGT→ACT	S→T
		579c	CAT→CGT	H→R
		773d	CAC→TAC	H→Y
U-373 MG	Persistent (passage 25)	102c	GAT→GTT	D→V
		279c	AGT→ACT	S→T
		579c	CAT→CGT	H→R
		752	TAT→CAT	Y→H
MO3.13	Persistent (passage 24)	474e	ACA→ATA	T→I
		579c	CAT→CGT	H→R
		752f	TAT→CAT	Y→H
		1488	GCT→GTT	A→V
		1496	AAT→CAT	N→H
		1811	TTT→ATT	F→I
		2079	TTT→TAT	F→Y

^aReference 53. 

^bReference 40. 

^cReference 39; isolates CU and VA. 

^dReference 39; isolate VA. 

^eReference 39; isolates CU and VA (a point mutation at the same position but for a different amino acid). 

^fPoint mutation observed in two out of three sequenced clones. 

^gOne H4 cell line demonstrated an in-frame deletion of 15 nucleotides (471 to 485), leading to a 5-amino-acid deletion.