Fig. 5. Gene transduction properties of AAV2.1-A, AAV1, AAV2, AAV5, and AAV9 vectors at their injection sites in rat cerebral cortex.

a mKO1 native-fluorescence (red) and NeuN immunofluorescence (green) at each vector injection site. Images are representative of n = 4 animals per group. Scale bar, 500 µm. b Comparison of mKO1 native-fluorescence intensity within a 1-mm-diameter circular observation window at the injection sites of the AAV2.1-A, AAV1, AAV2, AAV5, and AAV9 vectors. Data are expressed as the averaged value obtained in four rats, relative to the mean for the AAV2-CMV vector defined as 1.0. Each dot represents the intensity value for each individual. c Comparison of mKO1-positive neuron number obtained from stereological cell counts. The cell counts were done over the whole transgene-expressing region in each section. See the Methods for the details of fluorescence intensity and cell-count analyses. Each dot represents the neuron number for each individual. d Ratios of cells double-labeled for NeuN to the total mKO1-positive cells at each vector injection site. Each ratio was determined as the mean of data obtained in three sections examined, including the section through the center of the injection site. Each dot represents the ratio for each individual. e Ratios of cells double-labeled for S100β to the total mKO1-positive cells. Each ratio was determined as above. Each dot represents the ratio for each individual. Significant difference from the AAV2.1 vector (one-way ANOVA followed by post hoc Dunnett’s test): *P < 0.05, **P < 0.01, ***P < 0.001, n.s. P > 0.05. Source data are provided with this paper. The exact P values are also available as a Source Data file. Error bars, SEM. n = 4 biologically independent animals.