Fig. 7. Histological analyses of striatal sections subjected to chemogenetic manipulation.

a, b Sites of vector injections immunostained with anti-GFP (a) or anti-M3 (b) antibody in Monkeys E and F, respectively. Coronal sections. Scale bar, 1 mm. cc corpus callosum, Cd caudate nucleus, GPe external segment of the globus pallidus, GPi internal segment of the globus pallidus, ic internal capsule, LV lateral ventricle, Put putamen. c, d Immunostaining for c-fos at individual vector injection sites corresponding to a, b. Scale bar, 100 µm. e, f Number of c-fos-positive neurons within a 1-mm-diameter circular observation window at individual vector injection sites corresponding to a, b. Expressed as the mean value of cell counts obtained from three equidistant sections. The cell counts were done over the whole transgene-expressing region within the Cd or Put in each section. The averaged number of c-fos positive neurons at the densest area is chosen as a representative value. See the Methods for the details of the cell-count analysis. g GFP immunostaining showing anterograde labeling in the substantia nigra pars reticulata (SNr, upper) and thalamus (lower) on the side ipsilateral to the AAV2.1-A vector injection. Anterograde axonal labeling is also seen in the GPe and GPi in the lower-left panel of a. Only a single neuron is retrogradely labeled in the substantia nigra pars compacta (SNc). Data were obtained in Monkey E. CM centromedian thalamic nucleus, cp cerebral peduncle, Pf parafascicular thalamic nucleus. Scale bars, 100 µm. Source data are provided as a Source data file.