Table 1.
Animal ID | Serotype | % Capsid | Promoter | Gene | Stock titer (gc/ml) | Injection titer (gc/ml) | |
---|---|---|---|---|---|---|---|
AAV1 | AAV2 | ||||||
Monkey A, Monkey B | AAV2 | 0 | 100 | CMV Syn |
mKO1 mKO1 |
5.0 × 1013 8.0 × 1013 |
1.5 × 1013 6.0 × 1013 |
AAV2.1-A | 10 | 90 |
CMV Syn |
mKO1(i) mKO1(ii) |
2.2 × 1014 2.8 × 1014 |
1.5 × 1013 6.0 × 1013 |
|
AAV2.1-B | 50 | 50 |
CMV Syn |
mKO1 mKO1 |
9.3 × 1014 1.0 × 1015 |
1.5 × 1013 6.0 × 1013 |
|
AAV1 | 100 | 0 |
CMV Syn |
mKO1 mKO1(iii) |
7.7 × 1014 8.4 × 1014 |
1.5 × 1013 6.0 × 1013 |
|
Monkey C, Monkey D | AAV2.1-A | 10 | 90 |
CMV Syn |
mKO1 mKO1 |
s.a. (i) s.a. (ii) |
1.5 × 1013 6.0 × 1013 |
AAV1 | 100 | 0 |
CMV Syn |
mKO1 mKO1 |
8.5 × 1014 s.a. (iii) |
1.5 × 1013 6.0 × 1013 |
|
AAV5 | - | - |
CMV Syn |
mKO1 mKO1 |
5.0 × 1014 9.5 × 1014 |
1.5 × 1013 6.0 × 1013 |
|
AAV9 | - | - |
CMV Syn |
mKO1 mKO1 |
1.4 × 1015 1.3 × 1015 |
1.5 × 1013 6.0 × 1013 |
|
Monkey E | AAV2 | 0 | 100 | Syn | hM3Dq-IRES-AcGFP | 1.8 × 1013 | 1.0 × 1013 |
AAV2 | 0 | 100 | CMV | hM3Dq-IRES-AcGFP | 1.0 × 1013 | 1.0 × 1013 | |
AAV2.1-A | 10 | 90 | Syn | hM3Dq-IRES-AcGFP | 5.0 × 1013 |
1.0 × 1013 5.0 × 1013 |
|
Monkey F | AAV2 | 0 | 100 | Syn | hM3Dq | 1.0 × 1014 | 2.0 × 1013 |
AAV2 | 0 | 100 | Syn | hM3Dq-IRES-AcGFP | 2.0 × 1013 | 2.0 × 1013 | |
AAV2.1-A | 10 | 90 | Syn | hM3Dq-IRES-AcGFP | 4.0 × 1013 | 2.0 × 1013 | |
AAV1 | 100 | 0 | Syn | hM3Dq-IRES-AcGFP | 8.9 × 1013 | 2.0 × 1013 | |
Monkey G | AAV2.1-A | 10 | 90 | CaMKIIα | GCaMP6s(iv) | 1.2 × 1014 |
1.0 × 1014 2.0 × 1013 |
Syn | 1.2 × 1014 |
1.0 × 1014 2.0 × 1013 |
|||||
Monkey H | AAV2.1-A | 10 | 90 | CaMKIIα | GCaMP6s | s.a. (iv) | 4.0 × 1013 |
s.a., same as.