Table 5.
Genes regulated by 17β-estradiol (E; by analysis of variance, false discovery rate [FDR] ≤ 0.01) that correlated with percentage terminal ductal lobular units-1 (%TDLU1), %TDLU2, %TDLU3, number of terminal buds/field, and uptake of 5-bromo-2-deoxyuridine with an FDR of less than or equal to 0.01
| Gene names | Slope of %TDLU1 | Slope of %TDLU2 | Slope of %TDLU3 | Slope of No. of TBs | Slope of %BrDU+ | E-regulated P | Function |
|---|---|---|---|---|---|---|---|
| KPNA2 | –26.2 | 16.1 | 11.8 | 19.4 | 10.0 | 5.8E-08 | Nuclear protein transport |
| AURKA | –18.4 | 11.4 | 8.2 | 12.6 | 6.9 | 1.4E-08 | Spindle pole stabilization |
| BIRC5 | –20.3 | 12.5 | 9.1 | 13.0 | 7.4 | 2.0E-09 | Mitosis/Inhibits apoptosis |
| FANCD2 | –20.3 | 12.7 | 8.8 | 14.0 | 7.6 | 1.3E-08 | DNA repair |
| GINS1 | –15.7 | 9.9 | 7.0 | 11.0 | 5.9 | 1.1E-09 | DNA replication |
| LMNB2 | –29.7 | 18.8 | 12.7 | 21.8 | 10.7 | 3.9E-08 | Structure of nucleus |
| MAGED1 | –41.2 | 26.6 | 17.6 | 28.0 | 14.7 | 2.9E-09 | Programmed cell death |
| MCM4 | –22.6 | 15.0 | 9.4 | 13.9 | 8.1 | 6.4E-10 | DNA helicase |
| MCM6 | –24.5 | 16.0 | 10.2 | 17.1 | 9.1 | 1.5E-10 | DNA helicase |
| MTHFD2 | –30.9 | 20.1 | 13.3 | 19.6 | 10.9 | 1.9E-07 | One carbon metabolism |
| MYL7 | –16.9 | 10.8 | 7.4 | 10.6 | 6.2 | 4.9E-09 | Focal adhesion |
| PLK1 | –20.3 | 12.8 | 8.8 | 13.6 | 7.4 | 9.8E-09 | Mitosis |
| RAD54L | –23.3 | 14.4 | 10.5 | 15.8 | 8.5 | 8.5E-10 | DNA repair/recombination |
| SYCE2 | –33.8 | 20.2 | 15.4 | 21.0 | 12.8 | 2.0E-11 | Cell cycle |
| TSPAN7 | 24.6 | –15.3 | –11.4 | –16.3 | –9.1 | 1.3E-08 | Cell development, growth, activation, and motility |
Nonunderlined genes are positively correlated, and underlined genes are negatively correlated, with proliferation and development of TDLU-2, -3, and TB. Slope equals the linear regression slope for each gene.
Abbreviations: BrDU, 5-bromo-2-deoxyuridine; E, 17β-estradiol; FDR, false discovery rate; TDLU1, terminal ductal lobular units-1; TDLU2, terminal ductal lobular units-2; TDLU3, terminal ductal lobular units-3; TBs, terminal buds/field.