Figure 3.

mRNA encapsulated in LNP containing high levels of helper lipid exhibits improved stability properties when incubated with serum compared with free mRNA or mRNA encapsulated in Onpattro-type LNP formulations

LNP GFP mRNA systems containing 10% DSPC (DSPC/MC3/Chol/PEG-lipid 10:50:38.5:1.5 mol/mol) or 40% ESM (ESM/MC3/Chol/PEG-lipid 40:33:25.5:1.5 mol/mol) were prepared as described in methods (N/P = 6) and were incubated in 50% FBS at 37°C for 48 h mRNA stability was assessed using an Agilent 2100 Bioanalyzer (see methods). Data are representative of two separate experiments.