Figure 6.

LNP GFP mRNA systems containing 40 mol % ESM exhibit superior protein expression in liver (C57B16 mice) at 4 and 24 h post injection compared with systems containing 10 mol % ESM

Flow cytometry results showing LNP uptake and GFP expression in hepatocytes. For original flow data see Figure S3A. (A) Bar graphs represent the arithmetic mean ± SD of the percentage of DiD+ cells and (B) the arithmetic mean ± SD of the percentage of cells co-expressing DiD and GFP. ∗p < 0.05. The data represent three measurements of duplicate mice in three different experiments.