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. 2023 May 31;22(8):e13875. doi: 10.1111/acel.13875

FIGURE 2.

FIGURE 2

Effect of endothelial cell (EC) Trf2 reduction on telomere dysfunction, senescence, and endothelial function. (a) Trf2 mRNA expression in lung endothelial cells, carotid artery endothelial cells, perigonadal white adipose tissue (pgWAT), and skeletal muscle. (b) Percentage of endothelial cells containing one or more telomere dysfunction‐induced foci (TIF). (c) Number of telomere dysfunction‐induced foci (TIF) per endothelial cell. (d) Representative images of immunofluorescence fluorescent in situ hybridization performed on lung endothelial cells from WT and EC‐TRF2f/f mice. (e) Lung endothelial cell mRNA expression for senescence markers and SASP factors. (f) Carotid artery endothelial cell p21 mRNA expression. (g) Percentage of lung endothelial cells expressing senescence‐associated β‐galactosidase (SA‐βgal). (h) BrdU incorporation in lung endothelial cells. (i) Normalized transendothelial resistance, a measure of barrier function, of lung endothelial cells detected using electric cell‐substrate impedance sensing. (j) Lung endothelial cell migration distance from scratch migration assay. Scale bars 10 μm and 2 μm. N = 3–11 per group. Data are mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.