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. 2023 Jun 26;22(8):e13895. doi: 10.1111/acel.13895

FIGURE 3.

FIGURE 3

Tac effect on MAM‐related proteins in AβO‐treated cells. Schematic representation of proteins at MAM (a). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Relative expression of Insp3r (b), Sigmar1 (c), Grp75 (d), Vdac1 (e), Mcu (f), Pdzd8 (g), Vapb (h), Rmdn3 (i), Mfn2 (j), and Mfn1 (k) mRNA. Western blotting analysis of InsP3R1 (l), GRP75 (m), and VDAC1 (n) protein levels. Actin was used as the housekeeping messenger and the loading control. Data are the mean ± SEM of 3–6 independent experiments, run in triplicates to quadruplicates. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test, Mann–Whitney test and unpaired Student's t‐test; *p < 0.05; **p < 0.01 when compared to control.