Fig. 3.

WBP2 attenuated cisplatin-induced renal injuries. A H & E staining showed that cisplatin-induced renal morphological changes were attenuated by WBP2 overexpression but accentuated by WBP2 knockdown; B - E Renal functional analysis showed that WBP2 overexpression alleviated, while its gene disruption aggravated, cisplatin-induced increase in serum creatinine levels and BUN levels (n = 5; *P < 0.0001 compared with Ad-NC or Ad-shNC group, ^#P < 0.0001 compared with Ad-NC + CP or Ad-shNC + CP group, 1-way ANOVA with Dunn's multiple comparisons); F - G Western blot studies revealed that WBP2 overexpression attenuated cisplatin-induced upregulation of NGAL and KIM-1, while WBP2 knockdown presented the opposite effect; H - J Western blot studies showed that WBP2 was manipulated by the intraparenchymal injection of adenovirus, and GPF was also expressed in adenovirus-injected kidneys. CP: cisplatin, Ad-NC: empty vector adenovirus for WBP2 overexpression, Ad-WBP2: adenovirus-mediated WBP2 overexpression, Ad-shNC: empty vector adenovirus for WBP2 knockdown, Ad-shWBP2: adenovirus-mediated WBP2 knockdown. Data are presented as mean ± SD. Scale bars: 100 μm.