Fig. 6.

WBP2 and ferroptosis-related markers were depleted in the kidneys of AKI patients. A Immunohistochemistry staining showed that the expression of WBP2, GPX4, FTH1, and NCOA4 was decreased in the renal samples of ATN patients, as compared with the controls; B CK18 staining showed conceivable fluorescence in human primary tubular cells (HPT cells) and HK-2 cells, but no fluorescence was observed in HMC cells; C Cisplatin treatment led to decreased expression of WBP2, GPX4, FTH1, and NCOA4 in HPT cells. ATN: acute tubular necrosis, DMF: dimethylformamide, CP: cisplatin. Scale bars: 100 μm.