Table 2.
Table of Antibodies
| Category | Target | Host | Isotype [Clone] | Vendor | Catalog Number | Dilution | Conjugate |
|---|---|---|---|---|---|---|---|
| 1o Antibody | Col1 | Mouse | IgG1 [COL-1] | Abcam | AB6308 | 1:200 | |
| 1o Antibody | Col2 | Rabbit | Polyclonal | Invitrogen | PA5-85108 | 1:100 | |
| 1o Antibody | α-SMA | Mouse | IgG2a [1A4] | R&D Systems | IC1420S | 1:75 | Alexa 750 |
| 2o Antibody | Mouse IgG1 | Goat | Jackson Immunoresearch | 115-165-205 | 1:333 | Cy3 | |
| 2o Antibody | Mouse IgG2a | Goat | Jackson Immunoresearch | 115-605-206 | 1:333 | Alexa 647 |
| Category | Description | Vendor | Catalog Number | Notes |
|---|---|---|---|---|
| Ancillary | PowerVision IHC/ISH Super Blocking | Leica Biosystems | BB02-110 | Protein Block |
| Ancillary | Antibody Diluent | Leica Biosystems | AR9352 | |
| Ancillary | Fluoro-Gel II with DAPI | Electron Microscopy Sciences | 17985-51 | Mounting Media |
| Consumable | Glass coverslips, 25 × 60 × 1.5 mm | Leica Biosystems | 3800161 |