Figure 2.

In vitro antigen-specific recognition and function of anti-CLDN18.2 CAR-T cells. (A) CAR-T cells were cocultured with different target cells at a 1:1 E:T ratio, and 24 h later, IFN-γ, IL-2, and GM-CSF production in the supernatants was determined by a Bio-Plex Pro Reagent Kit. The results show the mean concentration (pg/ml) ± SD of triplicate wells from one representative of three independent experiments. (B) CAR-T cells were cocultured with claudin 18.1- or claudin 18.2-expressing target cells at various E:T ratios for 24 h Cytotoxicity was measured using the Bright-Glo™ luciferase assay system. The results show the mean killing ± SD of triplicate wells from one of three independent experiments. (C) Dynamic killing of CAR-T cells against target cells was monitored by the xCELLigence RTCA MP instrument system for 72–96 h in each round. The experiment was repeated independently three times with similar results. Statistical analyses were performed using one-way ANOVA with Tukey’s post-hoc correction test (A); statistical significance was defined as ns, not significant, P > 0.05. NC, negative control; PC, positive control.