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Induction of joint inflammation by LPS treatment. Ten micrograms of LPS was injected into individual joints. Three (B and G), 7 (C and H), 14 (D and I), and 30 (E and J) days later, synovial tissues were surgically removed, fixed, and stained with hematoxylin-eosin as described in Materials and Methods. (A and F) Tissues without LPS treatment. Arrows, polymorphonuclear cells. Magnifications, ×100 (A to E) and ×400 (F to J). Bar in panel A, 100 μm; bar in panel F, 10 μm.
