FIG. 4.

Reinduction of the rAAV-delivered gene by LPS treatment. Normal rat knee joints were injected with 10 μg of LPS. Twelve hours later, 10⁷ infectious units of rAAV-lacZ was injected into the same knee joints. Thirty days later, a second dose of 10 μg of LPS was injected into the same knees, and then 3 (B), 7 (C), 14 (D), and 30 (E) days after the second LPS treatment, synovial membranes were surgically removed, fixed, and stained for β-galactosidase (lacZ) activity. (A) Tissue from knees without the second LPS injection. Bar, 100 μm.