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. 2023 Jul 4;299(8):105013. doi: 10.1016/j.jbc.2023.105013

Figure 2.

Figure 2

VLCFA analysis in ACBD5 and ACBD4 KO HEK293 cells.A, analysis of levels of C26:0 and C26:0-lysoPC in control, ACBD4 and ACBD5 KO HEK293 cells. B, analysis of C26:0 and C16:0 β-oxidation (as a percentage relative to controls) in ACBD4, ACBD5 KO, and control HEK293 cells. C, D3-C22 loading assay with analysis of D3C26:0, D3C16:0, and the ratio of D3C16:0/D3C26:0 in control ACBD4 and ACBD5 KO HEK293 cells. Data analyzed by two-tailed unpaired t test; ns, not significant; ∗p ≤ 0.0332; ∗∗p ≤ 0.0021; ∗∗∗p ≤ 0.0002; ∗∗∗∗p ≤ 0.0001. Error bars represent SD, with three experiments per condition. KO, CRISPR knock-out.