Figure 5.

Substrate preference of ACBD4 and ACBD5.A, binding activity was measured with 2 μM protein and increasing concentrations of 14C-C18:1-CoA (0.5–20 μM). Error bars represent the standard deviations of at least three measurements. Competition of the binding of 14C-C18:1-CoA (5 μM) was performed with increasing concentrations of C16-CoA, C18:1-CoA and C24-CoA (0.2–20 μM), as indicated to (B) ACBD4 and (C) ACBD5. Control reactions were performed in the absence of the competitors and values obtained in their presence are presented relative to the values obtained in their absence. Error bars in the four plots represent the standard deviations of three measurements.