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. 2023 Jul 4;299(8):105013. doi: 10.1016/j.jbc.2023.105013

Figure 6.

Figure 6

Interactions between ACBD4 and ACBD5.A, FLAG-ACBD5, WT and FFAT mutants, and Myc-ACBD5, (B) FLAG-ACBD4, WT and FFAT mutants, and Myc-ACBD4, (C) FLAG-ACBD5, WT and CC mutants, and Myc-ACBD5, (D) FLAG-ACBD4, WT and CC mutants, and Myc-ACBD4 were expressed in COS-7 cells. Myc-ACBD4/5 was immunoprecipitated and bound FLAG-proteins detected by immunoblotting using FLAG/MYC antibodies. E, FLAG-ACBD4, WT, CC, and FFAT mutants, and Myc-ACBD5 were expressed in COS-7 cells. Myc-ACBD5 was immunoprecipitated and bound FLAG-proteins were detected by immunoblotting using FLAG/MYC antibodies. Inputs represent 1% of total lysate. CC, coiled-coil mutant; FFAT, FFAT mutant; IP, immunoprecipitation.