Fig. 1. CD2-iCre– or Lck-Cre–mediated deletion of Ptpn6 restores T cell development in Themis−/− mice.
(A) Western blotting analysis of SHP1 in total thymocytes from CD2-iCre or CD2-iCre;Ptpn6f/f mice (left) or in total thymocytes from Lck-Cre or Lck-Cre;Ptpn6f/f mice (right). Actin was used as a loading control. For all subsequent experiments, efficient Cre-mediated deletion was also confirmed, where appropriate, by Western blotting analysis. (B and C) Phenotypic comparison of thymocytes (left) and splenocytes (right) from representative CD2-iCre (B) or Lck-Cre (C) mice of the indicated genotype. Numbers indicate the percentage of cells within each gate. Data are representative of at least ten mice for each genotype.