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. Author manuscript; available in PMC: 2023 Aug 9.
Published in final edited form as: Sci Signal. 2023 May 9;16(784):eade1274. doi: 10.1126/scisignal.ade1274

Fig. 4. Overexpression of SHP1 results in a phenotype that closely resembles that of Themis−/− mice.

Fig. 4.

(A) Left: Western blotting analysis of the relative abundance of THEMIS in total thymocytes from Themis+/+ and Themis-Tg littermates. Right: Western blotting analysis of the relative abundance of SHP1 in total thymocytes from Ptpn6+/+ and Ptpn6-Tg littermates. The intensities of THEMIS and SHP1 bands from three experiments were quantitated by densitometry relative to those for actin bands. The value for WT (Themis+/+) cells was arbitrarily set at 100% and all results are shown relative to that. (B) Phenotypic comparison of thymocytes (left) and splenocytes (right) from representative mice of the indicated genotype. Numbers indicate the percentage of cells within each gate. (C) Effect of SHP1 overexpression on the developmental block in Themis+/− and Themis−/− thymocytes. Phenotypic comparison of thymocytes (left) and splenocytes (right) from representative mice of the indicated genotype. Numbers indicate the percentage of cells within each gate. Results are representative of at least ten mice for each genotype.