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. Author manuscript; available in PMC: 2023 Aug 9.
Published in final edited form as: Sci Signal. 2023 May 9;16(784):eade1274. doi: 10.1126/scisignal.ade1274

Fig. 5. Inhibition of the tyrosine phosphatase activity of SHP1 alleviates the block in positive selection in Themis−/− mice.

Fig. 5.

Gestation day 16 fetal thymus lobes were cultured in medium for 2 days and then for a further 3 days with medium alone or medium containing 40 nM TPI-1, 10 μM pervanadate (PV),, 5 mM N-acetyl-L-cysteine (NAC), or 4 mM L-buthionine-sulfoximine (BSO). Left: The thymocytes were then harvested and analyzed by flow cytometry. Numbers indicate the percentage of cells within each gate. Right: The percentages of CD4 SP thymocytes and the percentage of CD4 SP thymocytes divided by the percentage of DP thymocytes for the indicated treatments. Data are means ± SD of the indicated thymocyte subset percentages and were analyzed by two-tailed, type 2, t test. All comparisons are to untreated controls. *P < 0.05, **P < 0.01. Combined results are from four to six thymus lobes for each treatment. The genotype of fetal thymi was confirmed by PCR.