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. Author manuscript; available in PMC: 2023 Aug 9.
Published in final edited form as: Sci Signal. 2023 May 9;16(784):eade1274. doi: 10.1126/scisignal.ade1274

Fig. 6. Oxidation (inactivation) of SHP1 in thymocytes is enhanced by overexpression of THEMIS (Themis-Tg) and is diminished by deletion of Themis (Themis−/−).

Fig. 6.

(A and B) Effect of THEMIS overexpression (Themis-tg) or deletion (Themis−/−) on the oxidation of SHP1 by H2O2 (A) or pervanadate (B). Western blots show the analysis of cell lysates with antibodies against the indicated proteins. The antibody against oxidized PTP detects irreversibly oxidized (SO3H) phosphatases. Data are representative of three independent experiments (see figs. S6 and S7 for quantification data). (C and D) Effect of deletion of SHP1 (Ptpn6) (C) or overexpression of SHP1 (Ptpn6-Tg) (D) on TCR signaling. Total thymocytes from the indicated mice were incubated in complete medium for 6 hours at 37°C and then were stimulated with anti-CD3 and anti-CD4 antibodies for 0, 2, or 5 min. Western blotting analysis of cell lysates with antibodies against the indicated proteins. Data are representative of four (C) or three (D) independent experiments (see figs. S8 and S9 for data quantification).