Figure 11.

Induction of apoptosis of compound-treated PC3 cells were measured by flow cytometry after 24 h compound exposure. The compound-treated cells were stained dually with Annexin V-PE and PI prior to analysis. Lower left quadrant (both Annexine V and PI-negative fraction): intact cells. Lower right quadrant (Annexine V-positive/PI-negative fraction): early apoptosis. Upper right quadrant (Annexine V-positive/PI-positive fraction): late apoptosis. Upper left quadrant (Annexine V-negative/PI-positive fraction): necrosis. Staurosporine, an apoptosis inducer, was used as positive control.