Table 2. The Freeze-Drying Protocol Employed for Mb and LDH Formulations^*.

phase	description	temperature, °C	time, min	pressure, Pa
1	loading	20
2	cooling	–45	217
3	holding	–45	120
4	vacuum	–45		10
4	primary drying – ramp	–25	40	10
5	primary drying – holding	–25	*	10
6	secondary drying – ramp	20	240	10
7	secondary drying – holding	20	300	10

^*as indicated by the comparative Baratron/Pirani pressure measurement.²⁶