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. 2023 Jul 31;120(32):e2309967120. doi: 10.1073/pnas.2309967120

Fig. 1.

Fig. 1.

Activin E lowers fat mobilization and increases adiposity in mice. (A) Liver mRNA levels of Inhbe (n = 10). (B) Body weights in 7- to 14-wk-old male Control AAV and Inhbe AAV-treated WT mice (n = 10). (C) Weights of visceral (epididymal) and subcutaneous (inguinal) white adipose tissue (n = 10). (D) Adipocyte morphology and size distribution measured by imaging software in H&E-stained epididymal WAT sections (n = 534,046 cells for Control AAV and 525,314 cells for Inhbe AAV analyzed from 10 mice/group). (E) Ex vivo lipolysis with epididymal WAT explants from WT mice under basal and isoproterenol-stimulated conditions. Fat mobilization is determined enzymatically by glycerol measurements in supernatant (n = 5). (F) Plasma NEFA levels (n = 10). (G) Epididymal WAT mRNA levels of genes involved in adipose lipolysis, relative to Control AAV (n = 10). Mean ± SEM are shown in all graphs besides (D), where mean is shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 relative to Control AAV.