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. 2023 Jul 31;120(32):e2309967120. doi: 10.1073/pnas.2309967120

Fig. 3.

Fig. 3.

Loss of activin E promotes fat mobilization. (A) Body weights in 7- to 24-wk-old male WT and Inhbe−/− mice on chow and high-fat diet (HFD) (n = 7 to 8). (B and C) Weights of epididymal (B) and subcutaneous (C) white adipose tissue of Inhbe−/− mice following 16 wk of HFD (n = 8). (D) Ex vivo lipolysis with epididymal WAT explants from WT and Inhbe−/− mice under basal and isoproterenol-stimulated conditions (n = 10 to 11). (E and F) Fasted plasma NEFA (E) and beta-hydroxybutyrate (F) levels before (chow) and after 10 or 16 wk of HFD (n = 8). (G) Epididymal WAT mRNA levels of genes involved in adipose lipolysis (n = 6 to 10). (H and I) Fasted liver weight (H) and hepatic triglyceride content (I) in WT and Inhbe−/− mice on HFD (n = 8). (J) Oral glucose tolerance and insulin tolerance tests (K) in WT and Inhbe−/− mice on chow and high-fat diets (n = 7 to 8). (L) Plasma insulin levels before (baseline chow) and 16 wk after HFD (n = 8). (M) Plasma ALT levels in Inhbe−/− mice on chow and HFD for 16 wk. Mean ± SEM are shown in all graphs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.