Fig. 4.

Enhanced clustering strengthens light-induced membrane binding of BcLOV4. (A) The IDRs FUS(LC) and RGG were fused to BcLOV-mCh to test whether increasing BcLOV4 clustering strength could tune the magnitude of membrane binding. (B) Both IDR-fused variants of BcLOV-mCh showed dramatic enhancement in membrane translocation. (Scale bar, 20 μm.) See also Movie S5. (C) IDR-BcLOV fusions maintained strong membrane localization even after 2 h of stimulation at 37 °C, whereas wt BcLOV-mCh decays back to unstimulated levels. Data represent mean BcLOV4 membrane/cytoplasmic ratios of ~350 to 750 cells. Ribbons = 95% CI (see Methods section for quantification details). (D) The clustering strength of BcLOV4 can tune its ability to translocate to the membrane in response to light stimulation.