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. 2023 Jun 26;11(6):e006166. doi: 10.1136/jitc-2022-006166

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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G-MDSCs pomote the differentiation of M-MDSC into M2 macrophages. (A) Flow chart of inducing M-MDSCs to differentiate into M2 macrophages. (B) FACS analysis of the percentage of CD11b⁺Ly6C^hi cell and CD11b⁺Ly6C^low cell. (C) Summary graphs of the percentage of differentiated M-MDSCs (F4/80⁺Ly6C^low cells) to total M-MDSCs. (D) FACS analysis of sorted CD11b⁺F4/80⁺ cells. (E) Immunofluorescence analysis of CD206 in CD11b⁺F4/80⁺ cells. (F) T cell proliferation was detected using FACS. (G) Summary graphs of the percentage of proliferating T cells (n=3). (H) mRNA expression of cytokines in CD11b⁺F4/80⁺ cells was analyzed by qRT-PCR (n=3). (I) Representative results of tube formation (n=3). (J) Summary graphs of tube number (n=3). (K) The percentage of F4/80⁺Ly6C^low cell were detected by FACS (n=3). (L) Summary graphs of the percentage of differentiated M-MDSC to total M-MDSC (n=3). Statistical analyses were performed using unpaired t-tests. G-MDSCs, colitis-associated cancers; MDSC, myeloid-derived suppressor cell; M-MDSCs, monocytic MDSCs. (*p<0.05, **p<0.01, ***p<0.001,one-way ANOVA test; error bars, SD).