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. 2023 Jun 28;11(6):e006890. doi: 10.1136/jitc-2023-006890

Figure 1.

Figure 1

PRMT5 overexpression prevents the ferroptosis of TNBC cells. (A) After knockdown or overexpression of PRMT5, HCC1937 and MDA-MB-231 cells were treated with 0, 1, 2, 4, 8, and 16 μM erastin or 0, 0.5, 1, 2, 4, and 8 μM RSL3 for 24 hours. The killing efficiency was examined using the MTT assay. (B,C) PRMT5 overexpressing or PRMT5-deficient HCC1937 and MDA-MB-231 cells were treated with 3 μM RSL3 (3 μM) for 12 hours, and then BODIPY 581/591 C11 staining (B) and MDA assays (C) were conducted to detect the content of cellular lipid ROS (B) and MDA (C), respectively. (D) After 24 hours of treatment with 1 μM RSL3 in combination with 60 μM 3-MA, 10 μM Z-VAD-FMK, 10 μM necrostatin-1, or 1 μM Fer-1, the death of PRMT5-deficient HCC1937 and MDA-MB-231 cells was examined by flow cytometry. Average of three experiments. *p<0.05; **p<0.01; NS, no significance. PRMT5, protein arginine methyltransferase 5; TNBC, triple-negative breast cancer; DMSO, dimethyl sulfoxide; ROS, reactive oxygen species.