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. 2023 Aug 9;42:201. doi: 10.1186/s13046-023-02778-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — HPs CM disrupts P-FGFR, FAK and integrin α5β1 co-localization and induces YAP nuclear de-localization in MIAPaCa2 cells. (A) Immunofluorescence analysis of P-FGFR (green) and FAK (red) in MIAPaCa2 cells after 1 h of treatment with HPs CM ± 20 ng/mL of FGF2 (scale bar 10 μm). (B) Immunofluorescence of P-FGFR (green), integrin αvβ3 (red) and integrin α5β1 (red) in MIAPaCa2 cells (scale bar 10 μm). (C) Effect of anti-integrin αvβ3 and anti-integrin α5β1 antibodies (20 µg/mL) on adhesion of MIAPaCa2 cells treated with HPs CM (mean ± SEM, one-way ANOVA with Dunnett’s multiple comparison test, **p < 0.0001). (D) Immunofluorescence of YAP in MIAPaCa2 cells after 2 h of treatment with HPs CM ± 10 ng/mL of FGF2 (scale bar 25 μm). (E) Quantification of YAP localization (nucleus/cytoplasm ratio) (mean ± SEM; one-way ANOVA with Dunnett’s multiple comparison test, **p < 0.005,****p < 0.0001)