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. Author manuscript; available in PMC: 2023 Aug 9.
Published in final edited form as: Pediatr Allergy Immunol. 2010 Mar 19;21(4 Pt 1):640–648. doi: 10.1111/j.1399-3038.2010.01003.x

Fig. 5.

Fig. 5.

Kinetic changes in the mRNA expression of the Tm- & Sol-IL-5Rα isoforms in cord blood (CB) CD34+ cells incubated with IL-5 (shown as percent of total IL-5Rα). Non-adherent mononuclear cells (NAMNCs) from random fresh and frozen CB samples underwent magnet-activated cell separation using a CD34+ selection kit. CB CD34+ cells were incubated in the presence of rhIL-5, and removed at various time points for cell lysis, RNA extraction, reverse-transcription and determination of Tm-IL-5Rα and Sol-IL-5Rα mRNA expression patterns using SYBR green based quantitative polymerase chain reaction (Q-PCR) analysis.