. Author manuscript; available in PMC: 2023 Aug 9.

Published in final edited form as: Pediatr Allergy Immunol. 2010 Mar 19;21(4 Pt 1):640–648. doi: 10.1111/j.1399-3038.2010.01003.x

Table 1.

Primer/probe sequences for housekeeping genes and genes of interest

Gene	Sequence	Amplicon (bp)
Multiplex reactions
GAPDH	Probe: AAGGCTGAGAACGGG Forward primer: GACCTCAACTACATGGTTT Reverse primer: CTGGAAGATGGTGATGGGATTT	238
GATA-1	Probe: ACTCTCCCCCTGCCTC Forward primer: AGCCTATTCCTCTCCCAA Reverse primer: TCCGCAGTTCACACACTC	78
MBP1	Probe: GAGGAAGAGGAGGAGTG Forward primer: AGCAGGAGATGGAGGAGA Reverse primer: CTGGCACTGAGATAGACTCAAC	113
IL-5Rα	Probe: TTGGTTGTTGG^GAGTT Forward primer: TAATGGCTATCTGGACGAG Reverse primer: AAGCAAAT^AT^GGA^GGAGGA	110
SYBR green-based reactions (no probe required)
IL-5Rα soluble	Forward primer: GCAGCAGTGAGCTCCATGTG Reverse primer: TTCAGATACGGTGTGGGGCAG	124
IL-5Rα transmembrane	Forward primer: GCAGCAGTGAGCTCCATGTG Reverse primer: AGGGCTTGTGTTCATCATTTCCC	87
β-2-microglobulin	Forward primer: CCTGAATTGCTATGTGTCTGGG Reverse primer: TGATGCTGCTTACAGTCTCGA	245

Denotes superbases.