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. 2023 Jan 18;15(3):344–353. doi: 10.1055/s-0042-1760668

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The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ )

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 5 — Chromatograms of methotrexate (MTX) and p-aminoacetophenone (internal standard [IS]) in human serum: (A) blank serum; (B) blank serum spiked with MTX at 0.09 μmol/L and IS at 250 μmol/L; and (C) chromatograms of MTX and IS in a serum sample from a patient 24 hours after administration of MTX.