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. 2023 Jan 18;15(3):344–353. doi: 10.1055/s-0042-1760668

Table 2. Gradient method of liquid chromatography and total run time for each injection.

Time Flow (mL/min) % Mobile phase A % Mobile phase B
0.00 0.400 40.0 60.0
0.50 0.400 40.0 60.0
3.60 0.400 80.0 20.0
4.10 0.400 80.0 20.0
4.20 0.400 40.0 60.0
5.00 0.400 40.0 60.0
5.00 Stop run