FIG. 1.

Virus particle-associated material in the supernatant of transfected HeLa cells was analyzed by Western blotting with monoclonal antibodies that recognize the RT subunits p66 and p51 (A) or the gag-encoded MA and CA proteins (B). The top half of the Western blot shown in panel A was deliberately overexposed to allow the detection of the Gag-Pol precursor molecules. Arrows point to Gag-Pol cleavage intermediates. The molecular mass marker (in kilodaltons) is indicated for both panels A and B. Pre and post indicate that the viral protease was cloned from a pretherapy or postresistance patient isolate, respectively. neg, mock-transfected cells.