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. Author manuscript; available in PMC: 2024 Jun 8.
Published in final edited form as: Cell. 2023 Jun 8;186(12):2672–2689.e25. doi: 10.1016/j.cell.2023.05.019

Figure 1. Trivalent VLP immunization elicits single-specific and triple-specific α-EEV mAbs in NHP.

Figure 1.

(A) Cynomolgus macaque immunization regimen. (B) Percent of CD19+IgG+ B cells binding at least one VLP probe (left), and those binding all three VLP probes simultaneously (right). (C) Single B cells positive for binding only a single VLP (WEEV: red; EEEV: blue; VEEV: green) or all three VLPs (WEVEEV: black) were sorted into wells. A gray arrow denotes a doublet sort, comprising two B cells in one well (only one sequence was generated). Lineages were defined as having a unique V-gene allele, CDR3 sequence, and CDR3 length. (D) ELISA binding curves of single-specific and triple-specific ⍺-EEV mAbs to WEEV VLPs (top), EEEV VLPs (middle), and VEEV VLPs (bottom). Data are representative of two to three independent experiments. See also Figure S1 and Tables S1–S2.