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. 2023 Jul 16;19(12):3709–3725. doi: 10.7150/ijbs.84577

Figure 5.

Figure 5

AR/circ-SLCO1B7/miR-139-5p axis suppresses lung cancer cell progression via altering TPD52 expression. A. The distribution of 1922 upregulated genes on chromosomes in LUSC. B. The distribution of 1111 upregulated genes on chromosomes in LUAD. C. Overlapping genes were identified among the three groups. D-E. qRT-PCR assay was performed to screen genes in A549 cells transfected with shAR compared to pLKO (D) and in H1299 cells transfected with oeAR compared to the vector pWPI (E). F-I. Western blot assays were conducted to detect TPD52 expression in the two cell lines by manipulating AR/circ-SLCO1B7 and AR/miR-139-5p. J. Sequence alignment of the TPD52 3'UTR with wild-type and mutant sequences. K-L. Luciferase reporter activity was measured after transfection of wild-type or mutant TPD52 3'UTR reporter constructs in H1299 cells with or without oemiR-139-5p (K) and in A549 cells treated with or without miR-139-5p inhibitor (L). All quantitations are presented as mean ± SD, and statistical significance was calculated using the t-test. Significance levels are denoted as *p < 0.05, **p < 0.01, ***p < 0.001, and "ns" represents non-significance.