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. 2023 Jul 31;19(12):3892–3907. doi: 10.7150/ijbs.86636

Figure 2.

Figure 2

STX6 promotes HCC cell growth. (A and B) STX6 mRNA and protein level detection in STX6-overexpressing and STX6-deficient HCC cells. (C) Cell Counting Kit 8 assay of the proliferation of HCC cells with STX6 overexpression or knockdown. (D) Representative images of the EdU labeling assays of HCC cells with STX6 overexpression or knockdown. Scale bar: 100 µm. (E-G) Colony formation assay of HCC cells with STX6 overexpression or knockdown. (H) Flow cytometry analysis of the cell cycle MHCC-97H and Hep3B cells with STX6 knockdown and Huh7 and Li-7 cells with STX6 overexpression. (I) Tumor tissues inoculated from xenograft-bearing animals with STX6-overexpressing Huh7 and STX6-deficient MHCC-97H cell lines. (J) Quantitative data analysis of tumor weight and volume. Data for the in vitro experiments are the means ± SDs and are representative of three independent experiments. *p < 0.05, **p < 0.01 by two-tailed Student's t-test or one-way analysis of variance ANOVA. HCC, hepatocellular carcinoma; STX6, syntaxin-6.