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. 2023 Jul 31;19(12):3892–3907. doi: 10.7150/ijbs.86636

Figure 6.

Figure 6

STX6-overexpression sensitizes HCC cells to rapamycin by regulating autophagy. (A) Western blot analysis of STX6 and autophagy-related proteins in STX6-overexpressing HCC cells treated with rapamycin. (B) Cell Counting Kit-8 assay of the proliferation of STX6-overexpressing HCC treated with rapamycin. (C-D) Colony formation of STX6-overexpressing HCC cells treated with rapamycin. Bar graphs show the quantitative analysis of colony numbers. (E) Transwell invasion assay of STX6-overexpressing HCC cells treated with rapamycin. (F) Quantitative data analysis of Transwell assays to quantify the invasion of STX6-overexpressing HCC cells. (G) Expression of STX6 and LC3II/I in STX6 overexpressing HCC cells treated with rapamycin and HCQ. (H) Cell viability of STX6-overexpressing Li-7 and Huh7 cells treated with various concentrations of rapamycin (with or without 20 μM HCQ) for 72 h. (I) Long-term colony formation assays of STX6-overexpressing Huh7 and Li-7 cells treated with rapamycin. (J) Flow cytometric analysis of apoptosis of the apoptotic rate in STX6-overexpressing Li-7 and Huh7 treated with rapamycin. (K) Flow cytometric analysis of the apoptotic rate in STX6-overexpressing Li-7 and Huh7 treated with rapamycin and HCQ. Error bars represent the mean ± SD, n=3. * p < 0.05; ** p < 0.01 by two-tailed Student's t-test or one-way analysis of variance ANOVA. HCC, hepatocellular carcinoma; STX6, syntaxin-6.