Fig. 1.

Mob4 is essential for viability and male fertility in Drosophila melanogaster. a) Schematic of Mob4 gene and mutant alleles (red). The mob4^P deletion allele was generated by imprecise excision of the P-element in the line KG4509, and the mob4^SVC deletion allele was generated by CRISPR/Cas9-targeted mutagenesis. The endpoints of deletion in mob4^P and mob4^SVC were determined by sequencing and are indicated in basepairs (bp). b) Male fertility test of control (mCherry^RNAi), mob4^RNAi and rescued flies (mob4^RNAi and hmob4, both under UAS control). Males of each genotype were individually mated with wild-type females. Data points represent number of progeny from individual males. Means ± SEM are shown for at least n = 8 males per genotype. P-values of 2-tailed unpaired t-tests are indicated (99% confidence level); ****P < 0.0001, ***P < 0.001, ns, not significant. c) Fertility test of control and mob4^RNAi females. Females of each genotype were individually mated with wild-type males. Data points represent numbers of progeny from individual females. Means ± SEM are shown for n = 10 females per genotype. P-values of 2-tailed unpaired t-tests are indicated (99% confidence level). d) Viability of control, mob4^RNAi and rescued flies (as in b). Data points represent percentage of viable flies in 6 independent experiments. The da-GAL4 driver was crossed either to UAS-mCherry^RNAi (n = 439), UAS-mob4^RNAi (n = 338), UAS-mob4^RNAi; UAS-mCherry^RNAi (n = 201), or UAS-mob4^RNAi; UAS-hmob4 (n = 286). Means ± SEM are shown. P-values of 2-tailed unpaired t-tests are indicated (99% confidence level).