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. 2023 Jun 1;224(4):iyad104. doi: 10.1093/genetics/iyad104

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Fig. 2. — Individualization defects in mob4 depleted testes. Control (mCherry^RNAi) (a) and mob4^RNAi (d) testes from 2-day-old males were immunostained with antipolyglycylated tubulin (green) to visualize axonemes in individualizing/coiling cysts or mature sperm. The seminal vesicle (SV) and terminal epithelium (TE) are highlighted in white and pink, respectively. Control (b) and mob4^RNAi (e) testes were stained with phalloidin-594 and immunostained to reveal cleaved caspase 3 (cyan). Cystic bulges (CB, white arrows) and waste bags (WB, white arrowheads) resulting from spermatid individualization can be seen in control testes (b–c) but are absent from mob4-depleted testes (e–f). Actin cones and spermatid bundles from control (c) and mob4^RNAi (f) testes visualized with phalloidin-594 and immunostained to reveal polyglycylated tubulin (green).